American Society of Microbiology ABMM : ABMM

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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. For epidemiological tracking and outbreak investigations of Clostridioides difficile infection (CDI), which molecular typing method based on PCR amplification targeting repetitive elements within the pathogenicity locus (PaLoc) has historically been widely used and designated by specific nomenclature (e.g., NAP1/BI
/027)?

A) Whole-genome sequencing (WGS) SNP analysis
B) Pulsed-field gel electrophoresis (PFGE)
C) PCR Ribotyping
D) Multilocus sequence typing (MLST)

2. During an outbreak of norovirus gastroenteritis in a nursing home, environmental swabs are collected to identify the source of the infection. Which of the following methods would be MOST appropriate for detecting norovirus in these samples?

A) Enzyme-linked immunosorbent assay (ELISA) for viral antigens
B) Gram staining and microscopy
C) Culture on human embryonic kidney (HEK) cells
D) Reverse transcription-polymerase chain reaction (RT-PCR)

3. A clinical isolate of Klebsiella pneumoniae from a patient with a refractory urinary tract infection exhibits resistance to carbapenems (imipenem, meropenem, ertapenem). PCR analysis reveals the presence of the blaKPC gene. Which of the following enzymatic mechanisms is responsible for this resistance?

A) Hydrolysis of the beta-lactam ring, leading to an inactive antibiotic derivative.
B) Trapping of the carbapenem molecule by a decoy protein.
C) Efflux of the carbapenem antibiotic out of the bacterial cell.
D) Modification of the ribosomal target site, preventing antibiotic binding.

4. A research laboratory is investigating the mechanism of action of a novel antiviral compound that inhibits the replication of a specific RNA virus. They find that the compound binds directly to the viral RNA-dependent RNA polymerase, preventing its elongation activity. This mechanism is most similar to that of:

A) Oseltamivir
B) Interferon-beta
C) Acyclovir
D) Ribavirin

5. A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Streptococcus pneumoniae isolate recovered from a patient with meningitis. The isolate shows reduced susceptibility to penicillin (MIC = 2 µg/mL). The MOST likely mechanism for this reduced susceptibility is:

A) Modification of the peptidoglycan structure, preventing penicillin binding.
B) Increased activity of efflux pumps that actively remove penicillin from the cell.
C) Alterations in the penicillin-binding proteins (PBPs) that reduce their affinity for penicillin.
D) Production of beta-lactamase enzymes that hydrolyze penicillin.

Solutions: